![]() Progesterone levels increased during the reproductive cycle. ![]() These samples were classified according to reproductive stage, as identified by ultrasonographic examination, and the mean faecal progesterone and 17β-estradiol levels were calculated using the results from an enzyme-linked immunosorbent assay (ELISA). ![]() ![]() During the second reproductive cycle, the hormonal profiles of 30 animals were also evaluated, with a monthly collection of faecal samples. Ultrasound features, dimension and echogenicity of the reproductive structures were determined. We performed brief scans on non-anaesthetized snakes using a portable ultrasound system and a 10–12 MHz linear array transducer (MyLab™ 30 Gold, Esaote). regius was examined during two consecutive years. The reproductive activity of one hundred twenty-nine adult female P. The aim of the present study is to monitor the female reproductive cycle of the royal python using ultrasonography and gonadal steroid metabolite measurements in the faeces. However, there is limited information regarding the reproductive cycle of this species. For this purpose ultrasound has been anecdotally used to monitor royal python reproductive activity. The use of non-invasive monitoring methods in exotics is important in order to minimize stress. To have a successful breeding season, accurate monitoring of the reproductive activity is necessary. The royal python ( Python regius) is commonly bred in captivity.
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